Schedule 3 QUALITY AND ADDITIONAL SAMPLING REQUIREMENTS
| Parameter | Parametric value | Minimum sampling frequency | Methods of analysis and inspection |
|---|---|---|---|
| Notes
| |||
| Micro-biological: | |||
| Total coliforms | 10,000/100ml | Fortnightly (see Note 1) | Fermentation in multiple tubes. Sub-culturing of the positive tubes on a confirmation medium. Either counting according to MPN (most probable number) or membrane filtration, culturing on an appropriate medium, sub-culturing and identification of the suspect colonies. |
| Faecal coliforms | 2,000/100ml | Fortnightly (see Note 1) | The incubation temperature is variable according to whether total or faecal coliforms are being investigated. |
| Salmonella | Absent in 1 litre | (see Note 2) | Membrane filtration, culturing on an appropriate medium, sub-culturing and identification of the suspect colonies. |
| Entero viruses | No plaque forming units in 10 litres | (see Note 2) | Concentration (by filtration, flocculation or centrifuging) and confirmation. |
| Physico-chemical: | |||
| PH | 6 to 9 | (see Note 2) | Electrometry with calibration at pH 7 and 9. |
| Colour | No abnormal change in colour | Fortnightly (see Note 1) | Visual inspection or photometry with standards on the platinum cobalt scale. |
| Mineral oils | No film visible on the surface of the water and no odour | Fortnightly (see Note 1) | Visual and olfactory inspection. |
| Surface-active substances reacting with methylene blue | No lasting foam | Fortnightly (see Note 1) | Visual inspection. |
| Phenols (phenol indices) | No specific odour | Fortnightly (see Note 1) | Olfactory inspection. |
| ≤0.05 mg/litre (C6H5OH) | (see Note 2) | Absorption spectrophotometry 4-aminoantipyrine (4 AAP) | |
| Transparency | 1 metre | Fortnightly (see Note 1) | Secchi’s disc. |