{
  "url": "https://openlegislation.uk/bovine-embryo-collection-production-and-transfer-regulations-1995/schedule-7/paragraph-13",
  "json_url": "https://openlegislation.uk/bovine-embryo-collection-production-and-transfer-regulations-1995/schedule-7/paragraph-13.json",
  "legislation": "uksi/1995/2478",
  "legislation_title": "The Bovine Embryo (Collection, Production and Transfer) Regulations 1995",
  "legislation_url": "https://openlegislation.uk/bovine-embryo-collection-production-and-transfer-regulations-1995",
  "path": "schedule/7/paragraph/13",
  "kind": "paragraph",
  "number": "13",
  "heading": null,
  "text": "After the culture procedure has been completed, each bovine embryo shall be washed at least 10 times in a special fluid for bovine embryos which shall be changed each time (and which shall, for bovine embryos intended for national trade, contain trypsin, in accordance with internationally recognised procedures). Each wash shall be a 100-fold dilution of the previous wash and a sterile micropipette shall be used to transfer the bovine embryo on each occasion.",
  "extent": "E+W+S",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Word in Sch. 7 para. 13 substituted (31.12.2020) by The Trade in Animals and Related Products (Amendment) (EU Exit) Regulations 2019 (S.I. 2019/782), regs. 1, 5(19)(b); 2020 c. 1, Sch. 5 para. 1(1)"
    },
    {
      "type": "I",
      "label": "I1",
      "text": "Sch. 7 para. 13 in force at 13.10.1995, see reg. 1"
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/uksi/1995/2478/schedule/7/paragraph/13",
    "retrieved_at": "2026-10-29T13:01:18.978877+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}