{
  "url": "https://openlegislation.uk/draft-public-water-supplies-scotland-regulations-2014/schedule-3",
  "json_url": "https://openlegislation.uk/draft-public-water-supplies-scotland-regulations-2014/schedule-3.json",
  "legislation": "sdsi/2014/9780111024782",
  "legislation_title": "The Public Water Supplies (Scotland) Regulations 2014",
  "legislation_url": "https://openlegislation.uk/draft-public-water-supplies-scotland-regulations-2014",
  "path": "schedule/3",
  "kind": "schedule",
  "number": "3",
  "heading": "ANALYSIS: METHODS AND CAPABILITIES",
  "text": "TABLE M1\nSPECIFIED PARAMETERS: ANALYTICAL METHOD TO BE USED\n(1)\nItem\n(2)\nParameter\n(3)\nMethod\n(a)The composition of m-CP agar is:\nBasal medium—\nDissolve the ingredients of the basal medium, adjust pH to 7.6 and autoclave at 121°C for 15 minutes.\nTryptose\n30.0 g\nYeast extract\n20.0 g\nSucrose\n5.0 g\nL-cysteine hydrochloride\n1.0 g\nMgSO4.7H2O\n0.1 g\nBromocresol purple\n40.0 mg\nAgar\n15.0 g\nWater\n1,000.0 ml\nAllow the medium to cool and add—\nD-cycloserine\n400.0 mg\nPolymyxine-B sulphate\n25.0 mg\nIndoxyl-(beta)-D-glucoside to be dissolved in 8ml sterile water before addition\n60.0 mg\nFilter-sterilised 0.5% phenolphthalein disphosphate solution\n20.0 ml\nFilter-sterilised 4.5% FeCl3.6H2O\n2.0 ml\n1.\nClostridium perfringens (including spores)\nMembrane filtration followed by anaerobic incubation of the membrane on m-CP agar at 44 ± 1°C for 21 ± 3 hours. Count opaque yellow colonies that turn pink or red after exposure to ammonium hydroxide vapours for 20 to 30 seconds.\n2.\nColiform bacteria\nISO 9308-1\n3.\nColony count 22°C — enumeration of culturable microorganisms\nprEN ISO 6222\n4.\nColony count 37°C — enumeration of culturable microorganisms\nprEN 6222\n5.\nEnterococci\nISO 7899-2\n6.\nEscherichia coli\nISO 9308-1\nNotes—\nTABLE M2\nOTHER PARAMETERS: CAPABILITIES REQUIRED OF ANALYTICAL METHOD\n(1) Item\n(2) Parameter\n(3) Trueness % of prescribed concentration or value\n(4) Precision % of prescribed concentration or value\n(5) Limit of detection % of prescribed concentration or value\nNotes—\n(a)The method of analysis should determine total cyanide in all forms.\n(b)The capabilities required apply in relation to each individual pesticide.\n(c)The capabilities required apply in relation to each individual substance comprising PAH: Total at 25% of the prescribed concentration or value for PAH: Total.\n(d)The capabilities required apply at 50% of the prescribed concentration or value for PAH: Total.\n(e)The capabilities required apply in relation to prescribed concentration or value in Table B.\n(f)The capabilities required apply in relation to prescribed concentration or value in Table C.\nIn this Schedule “Pesticide”, “PAH: Total” and “THM: Total” have the meanings given in Schedule 1.\n1.\nAluminium\n10\n10\n10\n2.\nAmmonium\n10\n10\n10\n3.\nAntimony\n25\n25\n25\n4.\nArsenic\n10\n10\n10\n5.\nBenzene\n25\n25\n25\n6.\nBenzo(a)pyrene\n25\n25\n25\n7.\nBoron\n10\n10\n10\n8.\nBromate\n25\n25\n25\n9.\nCadmium\n10\n10\n10\n10.\nChloride\n10\n10\n10\n11.\nChromium\n10\n10\n10\n12.\nColour\n10\n10\n10\n13.\nConductivity\n10\n10\n10\n14.\nCopper\n10\n10\n10\n15.\nCyanide\n10\n10\n10\n16.\n1,2-dichloroethane\n25\n25\n10\n17.\nFluoride\n10\n10\n10\n18.\nIron\n10\n10\n10\n19.\nLead\n10\n10\n10\n20.\nManganese\n10\n10\n10\n21.\nMercury\n20\n10\n20\n22.\nNickel\n10\n10\n10\n23.\nNitrate\n10\n10\n10\n24.\nNitrite\n10\n10\n10\n25.\nPesticide\n25\n25\n25\n26.\nPAH: Total\n25\n25\n25\n27.\nSelenium\n10\n10\n10\n28.\nSodium\n10\n10\n10\n29.\nSulphate\n10\n10\n10\n30.\nTetrachloroethene\n25\n25\n10\n31.\nTetrachloromethane\n20\n20\n20\n32.\nTrichloroethene\n25\n25\n10\n33.\nTHM: Total\n25\n25\n10\n34.\nTurbidity\n10\n10\n10\n35.\nTurbidity\n25\n25\n25",
  "extent": null,
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "footnote",
      "label": "f1",
      "text": "(a)The composition of m-CP agar is: Basal medium— Dissolve the ingredients of the basal medium, adjust pH to 7.6 and autoclave at 121°C for 15 minutes. Tryptose30.0 gYeast extract20.0 gSucrose5.0 gL-cysteine hydrochloride1.0 gMgSO4.7H2O0.1 gBromocresol purple40.0 mgAgar15.0 gWater1,000.0 ml Allow the medium to cool and add— D-cycloserine400.0 mgPolymyxine-B sulphate25.0 mgIndoxyl-(beta)-D-glucoside to be dissolved in 8ml sterile water before addition60.0 mgFilter-sterilised 0.5% phenolphthalein disphosphate solution20.0 mlFilter-sterilised 4.5% FeCl3.6H2O2.0 ml"
    },
    {
      "type": "footnote",
      "label": "f2",
      "text": "(a)The method of analysis should determine total cyanide in all forms."
    },
    {
      "type": "footnote",
      "label": "f3",
      "text": "(b)The capabilities required apply in relation to each individual pesticide."
    },
    {
      "type": "footnote",
      "label": "f4",
      "text": "(c)The capabilities required apply in relation to each individual substance comprising PAH: Total at 25% of the prescribed concentration or value for PAH: Total."
    },
    {
      "type": "footnote",
      "label": "f5",
      "text": "(d)The capabilities required apply at 50% of the prescribed concentration or value for PAH: Total."
    },
    {
      "type": "footnote",
      "label": "f6",
      "text": "(e)The capabilities required apply in relation to prescribed concentration or value in Table B."
    },
    {
      "type": "footnote",
      "label": "f7",
      "text": "(f)The capabilities required apply in relation to prescribed concentration or value in Table C."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/sdsi/2014/9780111024782/schedule/3",
    "retrieved_at": "2026-10-30T15:35:33.542827+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}