# The Public Water Supplies (Scotland) Regulations 2014, Schedule 3: ANALYSIS: METHODS AND CAPABILITIES


TABLE M1
SPECIFIED PARAMETERS: ANALYTICAL METHOD TO BE USED
(1)
Item
(2)
Parameter
(3)
Method
(a)The composition of m-CP agar is:
Basal medium—
Dissolve the ingredients of the basal medium, adjust pH to 7.6 and autoclave at 121°C for 15 minutes.
Tryptose
30.0 g
Yeast extract
20.0 g
Sucrose
5.0 g
L-cysteine hydrochloride
1.0 g
MgSO4.7H2O
0.1 g
Bromocresol purple
40.0 mg
Agar
15.0 g
Water
1,000.0 ml
Allow the medium to cool and add—
D-cycloserine
400.0 mg
Polymyxine-B sulphate
25.0 mg
Indoxyl-(beta)-D-glucoside to be dissolved in 8ml sterile water before addition
60.0 mg
Filter-sterilised 0.5% phenolphthalein disphosphate solution
20.0 ml
Filter-sterilised 4.5% FeCl3.6H2O
2.0 ml
1.
Clostridium perfringens (including spores)
Membrane filtration followed by anaerobic incubation of the membrane on m-CP agar at 44 ± 1°C for 21 ± 3 hours. Count opaque yellow colonies that turn pink or red after exposure to ammonium hydroxide vapours for 20 to 30 seconds.
2.
Coliform bacteria
ISO 9308-1
3.
Colony count 22°C — enumeration of culturable microorganisms
prEN ISO 6222
4.
Colony count 37°C — enumeration of culturable microorganisms
prEN 6222
5.
Enterococci
ISO 7899-2
6.
Escherichia coli
ISO 9308-1
Notes—
TABLE M2
OTHER PARAMETERS: CAPABILITIES REQUIRED OF ANALYTICAL METHOD
(1) Item
(2) Parameter
(3) Trueness % of prescribed concentration or value
(4) Precision % of prescribed concentration or value
(5) Limit of detection % of prescribed concentration or value
Notes—
(a)The method of analysis should determine total cyanide in all forms.
(b)The capabilities required apply in relation to each individual pesticide.
(c)The capabilities required apply in relation to each individual substance comprising PAH: Total at 25% of the prescribed concentration or value for PAH: Total.
(d)The capabilities required apply at 50% of the prescribed concentration or value for PAH: Total.
(e)The capabilities required apply in relation to prescribed concentration or value in Table B.
(f)The capabilities required apply in relation to prescribed concentration or value in Table C.
In this Schedule “Pesticide”, “PAH: Total” and “THM: Total” have the meanings given in Schedule 1.
1.
Aluminium
10
10
10
2.
Ammonium
10
10
10
3.
Antimony
25
25
25
4.
Arsenic
10
10
10
5.
Benzene
25
25
25
6.
Benzo(a)pyrene
25
25
25
7.
Boron
10
10
10
8.
Bromate
25
25
25
9.
Cadmium
10
10
10
10.
Chloride
10
10
10
11.
Chromium
10
10
10
12.
Colour
10
10
10
13.
Conductivity
10
10
10
14.
Copper
10
10
10
15.
Cyanide
10
10
10
16.
1,2-dichloroethane
25
25
10
17.
Fluoride
10
10
10
18.
Iron
10
10
10
19.
Lead
10
10
10
20.
Manganese
10
10
10
21.
Mercury
20
10
20
22.
Nickel
10
10
10
23.
Nitrate
10
10
10
24.
Nitrite
10
10
10
25.
Pesticide
25
25
25
26.
PAH: Total
25
25
25
27.
Selenium
10
10
10
28.
Sodium
10
10
10
29.
Sulphate
10
10
10
30.
Tetrachloroethene
25
25
10
31.
Tetrachloromethane
20
20
20
32.
Trichloroethene
25
25
10
33.
THM: Total
25
25
10
34.
Turbidity
10
10
10
35.
Turbidity
25
25
25

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Source: https://www.legislation.gov.uk/sdsi/2014/9780111024782/schedule/3 (the official version)
Page: https://openlegislation.uk/draft-public-water-supplies-scotland-regulations-2014/schedule-3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.