Paragraph 5 Procedure
The test shall be carried out according to the following procedure:
the sample shall be at room temperature immediately before the test;
15.0 g of the sample shall be put into a small flask, 2.0 ml of the starch solution described in paragraph 3(a) above shall be added and mixed thoroughly;
the mixture shall be placed for 30 minutes in a water bath maintained at 44°C ± 0.5°C and anchored securely; then removed and cooled to room temperature;
5 ml of the mixture shall be added to 5 ml of trichloroacetic acid solution in a test tube fitted with a ground glass joint and suitable stopper (or an equivalent item of equipment) and shaken thoroughly;
15 ml of water shall be added and the mixture shaken again; then it shall be filtered and if the solution is cloudy or turbid, the first runnings shall be rejected;
10 ml of the clear filtrate shall be added to 2 ml of iodine solution contained in a test tube.