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  "json_url": "https://openlegislation.uk/eu-decision-1992-608.json",
  "id": "eudn/1992/608",
  "title": "Council Decision of 14 November 1992 laying down methods for the analysis and testing of heat-treated milk for direct human consumption (92/608/EEC)",
  "short_title": "Council Decision of 14 November 1992 laying down methods for the analysis and testing of heat-treated milk for direct human consumption (92/608/EEC)",
  "long_title": "Council Decision of 14 November 1992 laying down methods for the analysis and testing of heat-treated milk for direct human consumption (92/608/EEC)",
  "citation": "EU Decision 1992/608",
  "type": "eudn",
  "year": "1992",
  "number": "608",
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      "number": "6.5.10",
      "heading": null,
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-II/division-6/division-6.5/division-6.5.10",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/II/division/6/division/6.5"
    },
    {
      "path": "annex/2/division/II/division/6/division/6.5/division/6.5.11",
      "kind": "division",
      "number": "6.5.11",
      "heading": "Carry out a third extraction by further repeating the operations described in 6.5.3. to 6.5.8. inclusive, but using only 15 ml of diethyl ether (4.4.) and 15 ml of light petroleum (4.5.); use the ether to rinse the inside of the neck of the extraction flask. If necessary, raise the interface to the middle of the neck of the flask to enable the final decantation of solvent to be as complete as possible.",
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-II/division-6/division-6.5/division-6.5.11",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/II/division/6/division/6.5"
    },
    {
      "path": "annex/2/division/II/division/6/division/6.5/division/6.5.12",
      "kind": "division",
      "number": "6.5.12",
      "heading": null,
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-II/division-6/division-6.5/division-6.5.12",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/II/division/6/division/6.5"
    },
    {
      "path": "annex/2/division/II/division/6/division/6.5/division/6.5.13",
      "kind": "division",
      "number": "6.5.13",
      "heading": "Heat the fat-collecting vessel (with the flask placed on its side to allow solvent vapour to escape) for one hour in the oven (5.4.). Remove the fat-collecting vessel from the oven, allow to cool (not in a desiccator, but protected from dust) to the temperature of the weighing room (for glass vessels allow at least one hour, for metal dishes allow at least 30 minutes) and weigh to the nearest 0,1 mg.",
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-II/division-6/division-6.5/division-6.5.13",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/II/division/6/division/6.5"
    },
    {
      "path": "annex/2/division/II/division/6/division/6.5/division/6.5.14",
      "kind": "division",
      "number": "6.5.14",
      "heading": null,
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-II/division-6/division-6.5/division-6.5.14",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/II/division/6/division/6.5"
    },
    {
      "path": "annex/2/division/II/division/6/division/6.5/division/6.5.15",
      "kind": "division",
      "number": "6.5.15",
      "heading": "Add 25 ml of light petroleum to the fat-collecting vessel in order to verify whether or not the extracted matter is wholly soluble. Warm gently and swirl the solvent until all the fat is dissolved.",
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-II/division-6/division-6.5/division-6.5.15",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/II/division/6/division/6.5"
    },
    {
      "path": "annex/2/division/II/division/6/division/6.5/division/6.5.16",
      "kind": "division",
      "number": "6.5.16",
      "heading": "If the extracted matter is not wholly soluble in the light petroleum, or in case of doubt, extract the fat completely from the vessel by repeatedly washing with warm light petroleum.",
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-II/division-6/division-6.5/division-6.5.16",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/II/division/6/division/6.5"
    },
    {
      "path": "annex/2/division/II/division/7",
      "kind": "division",
      "number": "7",
      "heading": "EXPRESSION OF RESULTS",
      "url": null,
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/II"
    },
    {
      "path": "annex/2/division/II/division/7/division/7.1",
      "kind": "division",
      "number": "7.1",
      "heading": "Calculation and formula",
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-II/division-7/division-7.1",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/II/division/7"
    },
    {
      "path": "annex/2/division/II/division/7/division/7.2",
      "kind": "division",
      "number": "7.2",
      "heading": "Precision",
      "url": null,
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/II/division/7"
    },
    {
      "path": "annex/2/division/II/division/7/division/7.2/division/7.2.1",
      "kind": "division",
      "number": "7.2.1",
      "heading": "Repeatability (r):",
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-II/division-7/division-7.2/division-7.2.1",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/II/division/7/division/7.2"
    },
    {
      "path": "annex/2/division/II/division/7/division/7.2/division/7.2.2",
      "kind": "division",
      "number": "7.2.2",
      "heading": "Reproducibility (R):",
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-II/division-7/division-7.2/division-7.2.2",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/II/division/7/division/7.2"
    },
    {
      "path": "annex/2/division/III",
      "kind": "division",
      "number": "III",
      "heading": "DETERMINATION OF TOTAL NON-FAT SOLIDS",
      "url": null,
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2"
    },
    {
      "path": "annex/2/division/III/division/1",
      "kind": "division",
      "number": "1",
      "heading": "SCOPE AND FIELD OF APPLICATION",
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-III/division-1",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/III"
    },
    {
      "path": "annex/2/division/III/division/2",
      "kind": "division",
      "number": "2",
      "heading": "DEFINITION AND CALCULATION",
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-III/division-2",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/III"
    },
    {
      "path": "annex/2/division/IV",
      "kind": "division",
      "number": "IV",
      "heading": "DETERMINATION OF TOTAL NITROGEN CONTENT",
      "url": null,
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2"
    },
    {
      "path": "annex/2/division/IV/division/1",
      "kind": "division",
      "number": "1",
      "heading": "SCOPE AND FIELD OF APPLICATION",
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-1",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV"
    },
    {
      "path": "annex/2/division/IV/division/2",
      "kind": "division",
      "number": "2",
      "heading": "DEFINITION",
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-2",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV"
    },
    {
      "path": "annex/2/division/IV/division/3",
      "kind": "division",
      "number": "3",
      "heading": "PRINCIPLE",
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-3",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV"
    },
    {
      "path": "annex/2/division/IV/division/4",
      "kind": "division",
      "number": "4",
      "heading": "REAGENTS",
      "url": null,
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV"
    },
    {
      "path": "annex/2/division/IV/division/4/division/4.1",
      "kind": "division",
      "number": "4.1",
      "heading": null,
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-4/division-4.1",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/4"
    },
    {
      "path": "annex/2/division/IV/division/4/division/4.2",
      "kind": "division",
      "number": "4.2",
      "heading": null,
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-4/division-4.2",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/4"
    },
    {
      "path": "annex/2/division/IV/division/4/division/4.3",
      "kind": "division",
      "number": "4.3",
      "heading": null,
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-4/division-4.3",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/4"
    },
    {
      "path": "annex/2/division/IV/division/4/division/4.4",
      "kind": "division",
      "number": "4.4",
      "heading": "Sodium hydroxide solution, 47% (m/m) 704 g NaOH/1 (20 oC).",
      "url": null,
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/4"
    },
    {
      "path": "annex/2/division/IV/division/4/division/4.4/annotation/1",
      "kind": "division",
      "number": null,
      "heading": null,
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-4/division-4.4/annotation/1",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/4/division/4.4"
    },
    {
      "path": "annex/2/division/IV/division/4/division/4.5",
      "kind": "division",
      "number": "4.5",
      "heading": null,
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-4/division-4.5",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/4"
    },
    {
      "path": "annex/2/division/IV/division/4/division/4.6",
      "kind": "division",
      "number": "4.6",
      "heading": null,
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-4/division-4.6",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/4"
    },
    {
      "path": "annex/2/division/IV/division/4/division/4.7",
      "kind": "division",
      "number": "4.7",
      "heading": "Volumetric solution",
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-4/division-4.7",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/4"
    },
    {
      "path": "annex/2/division/IV/division/4/division/4.8",
      "kind": "division",
      "number": "4.8",
      "heading": null,
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-4/division-4.8",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/4"
    },
    {
      "path": "annex/2/division/IV/division/4/division/4.9",
      "kind": "division",
      "number": "4.9",
      "heading": null,
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-4/division-4.9",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/4"
    },
    {
      "path": "annex/2/division/IV/division/4/division/4.10",
      "kind": "division",
      "number": "4.10",
      "heading": "Tryptophan (C11H12N2O2), phenacetin (C10H7CH2CONH2) or lysine mono- or di-hydrochloride (C6H14N2O2 HCl or C6H14N2O2 · 2HCl).",
      "url": null,
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/4"
    },
    {
      "path": "annex/2/division/IV/division/4/division/4.10/annotation/1",
      "kind": "division",
      "number": null,
      "heading": null,
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-4/division-4.10/annotation/1",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/4/division/4.10"
    },
    {
      "path": "annex/2/division/IV/division/5",
      "kind": "division",
      "number": "5",
      "heading": "APPARATUS AND GLASSWARE",
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-5",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV"
    },
    {
      "path": "annex/2/division/IV/division/6",
      "kind": "division",
      "number": "6",
      "heading": "PROCEDURE",
      "url": null,
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV"
    },
    {
      "path": "annex/2/division/IV/division/6/division/6.1",
      "kind": "division",
      "number": "6.1",
      "heading": "To the Kjeldahl flask (5.1.) add boiling aid (5.2.) (eg. three glass beads), 15 g of potassium sulphate (4.1.), 1,0 ml of copper sulphate solution (4.2.), approximately 5 g of milk sample (weighed to the nearest 0,001 g) and 25 ml of sulphuric acid (4.3.). Use the acid to wash down any copper sulphate solution, potassium sulphate or milk on the neck of the flask, and gently mix the contents of the flask.",
      "url": null,
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/6"
    },
    {
      "path": "annex/2/division/IV/division/6/division/6.1/annotation/1",
      "kind": "division",
      "number": null,
      "heading": null,
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-6/division-6.1/annotation/1",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/6/division/6.1"
    },
    {
      "path": "annex/2/division/IV/division/6/division/6.2",
      "kind": "division",
      "number": "6.2",
      "heading": "Heat each Kjeldahl flask on the digestion apparatus (5.5.), very gently at first so that all the black froth stays within the bulb. When the initial frothing has ceased and copious white vapour appears, boil vigorously (acid vapour will condense half-way up the neck of the flask) until no black particles remain and until the digest becomes clear pale blue-green in colour. Then boil gently for at least 1,5 hours. Note the following requirements:",
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-6/division-6.2",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/6"
    },
    {
      "path": "annex/2/division/IV/division/6/division/6.3",
      "kind": "division",
      "number": "6.3",
      "heading": "When the Kjeldahl flasks are cool, add 300 ml of water (see note) to each so as to wash carefully down the neck of the flask, and mix the contents thoroughly ensuring that the crystals which have separated out are dissolved. Add some boiling aid (5.2.) to ensure uniform boiling. Then to each flask, add 70 ml of sodium hydroxide solution (4.4.) (see note) by gently pouring the solution down the inclined neck of the flask to form a bottom layer in the bulb; do not wet the top of the neck with the sodium hydroxide solution.",
      "url": null,
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/6"
    },
    {
      "path": "annex/2/division/IV/division/6/division/6.3/annotation/1",
      "kind": "division",
      "number": null,
      "heading": null,
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-6/division-6.3/annotation/1",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/6/division/6.3"
    },
    {
      "path": "annex/2/division/IV/division/6/division/6.4",
      "kind": "division",
      "number": "6.4",
      "heading": "Immediately connect each Kjeldahl flask to a distillation apparatus (5.6.). Ensure that the tip of the condenser outlet-tube is immersed in 50 ml of boric acid solution (4.5.) together with 0,20 ml (5 - 6 drops) of indicator solution (4.6.) all contained in a conical flask (5.8.). Swirl the contents of each Kjeldahl flask to mix thoroughly and boil, but gently at first to prevent excessive frothing. When 100 to 125 ml of distillate have been collected, lower each conical flask until the tip of the condenser outlet-tube is approximately 40 mm above the 200 ml mark. Continue each distillation until irregular boiling (‘bumping’) starts and then immediately stop the heating. Disconnect each Kjeldahl flask and rinse the tip of each condenser outlet-tube with a little water, collecting the rinsings in the conical flask. Note the following requirements:",
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-6/division-6.4",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/6"
    },
    {
      "path": "annex/2/division/IV/division/6/division/6.5",
      "kind": "division",
      "number": "6.5",
      "heading": "Titrate each distillate with standard volumetric solution (4.7.) until the pH is 4,6 ± 0,1, using a pH meter and if desired an automatic burette. Addition of an indicator helps to check whether the titration is proceeding correctly. Take each burette reading to the nearest 0.01 ml with the aid of a magnifying lens (5.10.) avoiding errors of parallax.",
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-6/division-6.5",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/6"
    },
    {
      "path": "annex/2/division/IV/division/6/division/6.6",
      "kind": "division",
      "number": "6.6",
      "heading": "Carry out a blank test according to 6.1. to 6.5. inclusive, taking 5 ml of distilled water together with about 0,1 g of sucrose (4.8.) through the procedure instead of the milk sample.",
      "url": null,
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/6"
    },
    {
      "path": "annex/2/division/IV/division/6/division/6.6/annotation/1",
      "kind": "division",
      "number": null,
      "heading": null,
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-6/division-6.6/annotation/1",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/6/division/6.6"
    },
    {
      "path": "annex/2/division/IV/division/6/division/6.7",
      "kind": "division",
      "number": "6.7",
      "heading": null,
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-6/division-6.7",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/6"
    },
    {
      "path": "annex/2/division/IV/division/6/division/6.7.1",
      "kind": "division",
      "number": "6.7.1",
      "heading": "Check that no loss of nitrogen occurs as a result of excessive heat or mechanical leaks during distillation, by using a test portion of 0,15 g of ammonium oxalate or sulphate (4.9.) weighed to the nearest 0,001 g together with 0,1 g of sucrose (4.8.).",
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-6/division-6.7.1",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/6"
    },
    {
      "path": "annex/2/division/IV/division/6/division/6.7.2",
      "kind": "division",
      "number": "6.7.2",
      "heading": null,
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-6/division-6.7.2",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV/division/6"
    },
    {
      "path": "annex/2/division/IV/division/7",
      "kind": "division",
      "number": "7",
      "heading": "SAFETY PRECAUTIONS",
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-7",
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV"
    },
    {
      "path": "annex/2/division/IV/division/8",
      "kind": "division",
      "number": "8",
      "heading": "EXPRESSION OF RESULTS",
      "url": null,
      "extent": "E+W+S+N.I.",
      "status": null,
      "parent": "annex/2/division/IV"
    },
    {
      "path": "annex/2/division/IV/division/8/division/8.1",
      "kind": "division",
      "number": "8.1",
      "heading": "Calculation and formula:",
      "url": "https://openlegislation.uk/eu-decision-1992-608/annex-2/division-IV/division-8/division-8.1",
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