{
  "url": "https://openlegislation.uk/eu-decision-1995-117/annex/division-4",
  "json_url": "https://openlegislation.uk/eu-decision-1995-117/annex/division-4.json",
  "legislation": "eudn/1995/117",
  "legislation_title": "Commission Decision of 30 March 1995 fixing the criteria for the testing of poultry for slaughter originating in a surveillance zone for Newcastle disease, in application of Article 5 (3) of Council Directive 91/494/EEC (Text with EEA relevance) (95/117/EC)",
  "legislation_url": "https://openlegislation.uk/eu-decision-1995-117",
  "path": "annex/division/4",
  "kind": "division",
  "number": "4",
  "heading": "Virus isolation in embryonated fowls' eggs",
  "text": "The clarified supernatent fluid should be inoculated in 0,2 ml amounts into the allantoic cavity of each of a minimum of four embryonated, fowls' eggs which have been incubated for 8 to 11 days. Ideally, these eggs should be obtained from a specific pathogen free flock, but when this is impracticable it is acceptable to use eggs obtained from a flock shown to be free of antibodies to Newcastle disease virus. The inoculated eggs are held at 37 oC and candled daily. Eggs with dead or dying embryos as they arise, and all remaining eggs four days after inoculation, should be chilled to 4 oC and the allantoic-amniotic fluids tested for haemagglutination activity.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudn/1995/117/annex/division/4",
    "retrieved_at": "2026-11-01T15:19:26.709731+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}