{
  "url": "https://openlegislation.uk/eu-decision-2000-428/annex/chapter-VII/division-A/division-1",
  "json_url": "https://openlegislation.uk/eu-decision-2000-428/annex/chapter-VII/division-A/division-1.json",
  "legislation": "eudn/2000/428",
  "legislation_title": "2000/428/EC: Commission Decision of 4 July 2000 establishing diagnostic procedures, sampling methods and criteria for the evaluation of the results of laboratory tests for the confirmation and differential diagnosis of swine vesicular disease (notified under document number C(2000) 1805) (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-decision-2000-428",
  "path": "annex/chapter/VII/division/A/division/1",
  "kind": "division",
  "number": "1",
  "heading": null,
  "text": "An indirect sandwich ELISA has replaced the complement fixation test as the method of choice for the detection of swine vesicular disease viral antigen. The test is the same as that used for foot-and-mouth disease diagnosis. The tests for the two diseases must be performed at the same time, unless foot-and-mouth disease has already been ruled out. It is recommended in particular on samples of epithelium or fluid from vesicular lesions, in which both swine vesicular disease and foot-and-mouth disease viruses can be present at high titers in acutely infected pigs and detected in a few hours.\nDuplicate rows in multiwell ELISA plates are coated with rabbit antiserum to swine vesicular disease virus and to each of the seven serotypes of foot and mouth disease virus. These are the trapping sera. Test sample suspensions are added to each of the rows. Appropriate controls are also included. Homologous guinea-pig detection serum is added in the respective rows at the next stage followed by rabbit anti-guinea pig serum conjugated to an enzyme such as horse-radish peroxidase. Extensive washing is carried out between each stage to remove unbound reagents. A positive reaction is indicated if there is a colour reaction on the addition of chromogen and substrate. With strong positive reactions this will be evident to the naked eye, but results can also be read spectrophotometrically, in which case an absorbance reading 0,1 above background indicates a positive reaction.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "footnote",
      "label": "f1",
      "text": "Positive ELISA results are associated with the presence of at least 105 TCID50 (tissue culture infectious doses) of virus in the sample."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudn/2000/428/annex/chapter/VII/division/A/division/1",
    "retrieved_at": "2026-11-01T15:18:49.012315+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}