# 2000/428/EC: Commission Decision of 4 July 2000 establishing diagnostic procedures, sampling methods and criteria for the evaluation of the results of laboratory tests for the confirmation and differential diagnosis of swine vesicular disease (notified under document number C(2000) 1805) (Text with EEA relevance), Division 2

Extent: E+W+S+N.I.

To isolate and grow virus, clarified epithelial suspension is inoculated onto monolayer cultures of IB-RS-2 cells. Two dilutions of epithelial suspension, one high (1/500) and one low (1/10) should be used to avoid interference with virus growth by interferon, the release of which will interfere with the growth of swine vesicular disease virus. For virus isolation only antibiotics are added to maintenance medium. For differential diagnosis from foot-and-mouth disease virus, primary bovine thyroid cells, or baby hamster kidney cells (BHK-21) must also be inoculated.

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Source: https://www.legislation.gov.uk/eudn/2000/428/annex/chapter/VII/division/B/division/2 (the official version)
Page: https://openlegislation.uk/eu-decision-2000-428/annex/chapter-VII/division-B/division-2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.