# 2000/428/EC: Commission Decision of 4 July 2000 establishing diagnostic procedures, sampling methods and criteria for the evaluation of the results of laboratory tests for the confirmation and differential diagnosis of swine vesicular disease (notified under document number C(2000) 1805) (Text with EEA relevance), Division 3

Extent: E+W+S+N.I.

If a cytopathic effect develops, the supernatant fluid must be harvested from positive cultures when the effect is complete and used in the ELISA for virus identification. Negative cultures must be inoculated on fresh tissue cultures at 48 or 72 hours and this blind passage examined up to 72 hours later. In the absence of cytopathic effect after a further blind passage the sample can be declared negative for the presence of live virus.

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Source: https://www.legislation.gov.uk/eudn/2000/428/annex/chapter/VII/division/B/division/3 (the official version)
Page: https://openlegislation.uk/eu-decision-2000-428/annex/chapter-VII/division-B/division-3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.