Division 1

Nucleic acid recognition methods can be used to detect swine vesicular disease viral genome in clinical material using the PCR and to establish relationships between isolates of swine vesicular disease virus by determining the nucleotide sequence of part of the genome. Techniques using the PCR have been developed to improve the sensitivity of diagnosis. Slightly different reverse transcriptase-PCR procedures have been described using primers corresponding to highly conserved regions in the 1C and 1D genes.