# 2000/428/EC: Commission Decision of 4 July 2000 establishing diagnostic procedures, sampling methods and criteria for the evaluation of the results of laboratory tests for the confirmation and differential diagnosis of swine vesicular disease (notified under document number C(2000) 1805) (Text with EEA relevance), Division 1

Extent: E+W+S+N.I.

The ELISA for detection of antibodies is a monoclonal antibody-based competitive ELISA. If the serum sample contains antibodies to swine vesicular disease virus, the binding of a selected peroxidase-conjugated monoclonal antibody to virus antigen will be inhibited.
In this ELISA the swine vesicular disease viral antigen is trapped to the solid phase using monoclonal antibodies; then the sera samples are incubated at appropriate dilution, followed by the addition of the peroxidase-conjugated monoclonal antibody. Then, the inhibition of the monoclonal antibody binding is measured by means of a substrate and chromogen.

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Source: https://www.legislation.gov.uk/eudn/2000/428/annex/chapter/VIII/division/B/division/1 (the official version)
Page: https://openlegislation.uk/eu-decision-2000-428/annex/chapter-VIII/division-B/division-1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.