# 2000/428/EC: Commission Decision of 4 July 2000 establishing diagnostic procedures, sampling methods and criteria for the evaluation of the results of laboratory tests for the confirmation and differential diagnosis of swine vesicular disease (notified under document number C(2000) 1805) (Text with EEA relevance), Division 1

Extent: E+W+S+N.I.

The VN test and the ELISA are the recommended serological tests. Chapter X lists the reference sera, which are available from the National reference laboratory in order that standardised serological tests are carried out ....
The VN test must be considered as the reference test, but has the disadvantage that it takes 2 to 3 days to complete and requires tissue culture facilities.
The ELISA is more rapid and can be more easily standardised. The monoclonal antibody competition ELISA is the most reliable swine vesicular disease antibody ELISA described to date. It is recommended as a screening test on a large number of samples.
However, the VN test must be used as confirmatory test when necessary, in particular after first detection of positive samples in a holding. Pigs positive by ELISA, but negative by VN test can be disregarded.

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Source: https://www.legislation.gov.uk/eudn/2000/428/annex/chapter/VIII/division/C/division/1 (the official version)
Page: https://openlegislation.uk/eu-decision-2000-428/annex/chapter-VIII/division-C/division-1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.