Chapter X Swine vesicular disease reference sera
United Kingdom
| Reference serum | Origin | Commentf1 |
|---|---|---|
These comments relate to the testing of individual pigs. For sero-surveillance the sensitivity of the test used should be taken into account. | ||
i.e. a serum with a titre sufficiently greater than the cut-off that it should always score positive by ELISA and VN test in repeated testing. | ||
| 1 | Normal pig serum (NPS) | Negative control serum. |
| 2 | Serum collected 21 days post infec- tion (dpi) from a pig infected with swine vesicular disease virus strain UKG 27/72 (neat) | Strong positive control serum. |
| 3 | A 1:10 dilution in NPS of a serum collected five dpi from a pig infected with swine vesicular disease strain Italy 8/94 | A low-positive serum from a pig soon after infection with a recent European isolate of swine vesicular disease virus. The serum has been diluted to give a low positive result in ELISA and VNT. |
| 4 | A 1:40 dilution of a serum collected 21 dpi from a pig infected with swine vesicular disease virus strain UKG 27/72 | A low-positive serum defining the lowest level of antibodies that EU National Reference Laboratories should consistently score positive by ELISA and virus neutralisation. Equivalent to serum RS 01-04-94f2 |
| 5 | Serum collected four dpi from a pig infected with swine vesicular disease virus strain UKG 27/72 (neat) | A low-positive serum from a pig soon after infection. |
| 6 | Serum collected five dpi from a pig infected with swine vesicular disease virus strain UKG 27/72 (neat) | A low-positive serum from a pig soon after infection. |
Annotations
Footnotes
- f1
These comments relate to the testing of individual pigs. For sero-surveillance the sensitivity of the test used should be taken into account.
- f2
i.e. a serum with a titre sufficiently greater than the cut-off that it should always score positive by ELISA and VN test in repeated testing.