# 2002/106/EC: Commission Decision of 1 February 2002 approving a Diagnostic Manual establishing diagnostic procedures, sampling methods and criteria for evaluation of the laboratory tests for the confirmation of classical swine fever (Text with EEA relevance) (notified under document number C(2002) 381), Chapter IX: Minimum safety requirements for classical swine fever laboratories

Extent: E+W+S+N.I.

Table 1
Principles of biological containment appropriate for diagnostic laboratories
Additional requirements
Minimal requirements
General environment
Normal atmospheric pressure
Double HEPA filtration of exhaust air.
Dedicated rooms, used exclusively for classical swine fever diagnostic procedures.
Normal atmospheric pressure.
Dedicated rooms limited to defined procedures.
Laboratory clothing
Complete change of clothes on entry.
Laboratory clothing used only in the classical swine fever virus unit.
Disposable gloves for all manipulations of infected material.
Clothing sterilised before removal from unit, or washed within unit.
Dedicated outer clothing used only in the classical swine fever virus unit.
Disposable gloves for all manipulations of infected material.
Outer clothing sterilised before removal from unit, or washed within unit.
Control of personnel
Entry to unit limited to named, trained personnel.
Wash and disinfect hands on leaving unit.
Personnel not permitted near pigs for 48 hours after leaving unit.
Entry to unit limited to named, trained personnel.
Wash and disinfect hands on leaving unit.
Personnel not permitted near pigs for 48 hours after leaving unit.
Equipment
Biological safety cabinet (Class I or II) used for all manipulations of live virus. Cabinet should have double HEPA filtration of exhaust air.
All equipment needed for laboratory procedures to be available within the dedicated laboratory suite.
Table 2
Bio-safety requirements for experimental animal rooms
Requirements
General environment
Negative pressure controlled ventilation.
Double HEPA filtration of exhaust air.
Facility for complete fumigation/disinfection at end of experiment.
All effluents treated to inactivate classical swine fever virus (heat or chemical).
Laboratory clothing
Complete change of clothes on entry.
Disposable gloves for all manipulations.
Clothing sterilised before removal from unit, or washed within unit.
Control of personnel
Entry to unit limited to named, trained personnel.
Full shower on exit from unit.
Personnel not permitted near pigs for 48 hours after leaving unit.
Equipment
All equipment required for animal procedures to be available within the unit.
All materials to be sterilised on removal from unit or, in the case of animal samples, to be double wrapped in leakproof container which is surface disinfected for transport to the classical swine fever laboratory.
Animals
All animals to be slaughtered before leaving the unit, post mortem examinations to be completed within the bio-safe area, and carcasses incinerated on completion of examinations.

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Source: https://www.legislation.gov.uk/eudn/2002/106/annex/chapter/IX (the official version)
Page: https://openlegislation.uk/eu-decision-2002-106/annex/chapter-IX
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.