# 2002/106/EC: Commission Decision of 1 February 2002 approving a Diagnostic Manual establishing diagnostic procedures, sampling methods and criteria for evaluation of the laboratory tests for the confirmation of classical swine fever (Text with EEA relevance) (notified under document number C(2002) 381), Division 1

Extent: E+W+S+N.I.

A discriminatory serological ELISA test (discriminatory test) is available to successfully distinguish pigs which have been vaccinated with marker vaccines, that induce antibodies only against the E2 glycoprotein of classical swine fever virus, from pigs which have been infected with the wild type of classical swine fever virus. This test is designed to detect antibodies against the glycoprotein Erns of classical swine fever virus. It is based on the principle that non-infected animals vaccinated with marker vaccines only produce antibodies against the glycoprotein E2 of classical swine fever virus, whilst animals infected with field virus react and produce antibodies against other virus antigens, too.
This discriminatory test is sensitive and specific . However, also pigs which have become infected with Pestiviruses other than classical swine fever virus, such as BVD virus and BD virus, will also react Erns-positive. Furthermore, the sensitivity of the test is not ideal, as some marker-vaccinated and then infected animals may not react Erns-positive.
The data currently available suggest that the discriminatory test cannot be reliably used to test serum samples from feral pigs.

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Source: https://www.legislation.gov.uk/eudn/2002/106/annex/chapter/VIII/division/A/division/1 (the official version)
Page: https://openlegislation.uk/eu-decision-2002-106/annex/chapter-VIII/division-A/division-1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.