# Commission Decision of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results (notified under document number C(2002) 3044) (Text with EEA relevance) (2002/657/EC), Division 2.3.5.3: Performance criteria for fluorimetric detection

Extent: E+W+S+N.I.

The performance criteria for LC methods have to be fulfilled.
This applies to molecules that exhibit native fluorescence and to molecules that exhibit fluorescence after either transformation or derivatisation. The selection of the excitation and emission wavelengths in combination with the chromatographic conditions shall be done in such a way to minimise the occurrence of interfering components in blank sample extracts.
The nearest peak maximum in the chromatogram shall be separated from the designated analyte peak by at least one full peak width at 10 % of the maximum height of the analyte peak.

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Source: https://www.legislation.gov.uk/eudn/2002/657/annex/chapter/2.3/division/2.3.5.3 (the official version)
Page: https://openlegislation.uk/eu-decision-2002-657/annex/chapter-2.3/division-2.3.5.3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.