{
  "url": "https://openlegislation.uk/eu-decision-2003-422/annex/chapter-VI/division-B/division-2",
  "json_url": "https://openlegislation.uk/eu-decision-2003-422/annex/chapter-VI/division-B/division-2.json",
  "legislation": "eudn/2003/422",
  "legislation_title": "2003/422/EC: Commission Decision of 26 May 2003 approving an African swine fever diagnostic manual (Text with EEA relevance) (notified under document number C(2003) 1696)",
  "legislation_url": "https://openlegislation.uk/eu-decision-2003-422",
  "path": "annex/chapter/VI/division/B/division/2",
  "kind": "division",
  "number": "2",
  "heading": null,
  "text": "The HAD technique is recommended for the identification of ASF virus isolates due to its high sensitivity and specificity. HAD is based on the capability of the ASF virus to replicate in pig macrophages and induce haemadsorption in the presence of pig erythrocytes. A characteristic \"rosette\" of erythrocytes develops around the infected macrophages. However, a small number of field ASF virus strains may not induce haemadsorption, but they produce a cytopathic effect. These strains may be specifically identified using the DIF test on the sediments of the cell cultures or by PCR.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudn/2003/422/annex/chapter/VI/division/B/division/2",
    "retrieved_at": "2026-11-01T15:18:22.461284+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}