# 2003/422/EC: Commission Decision of 26 May 2003 approving an African swine fever diagnostic manual (Text with EEA relevance) (notified under document number C(2003) 1696), Division 4

Extent: E+W+S+N.I.

Virus isolation and identification by HAD are recommended as a reference test for the confirmation of positive results of a prior ELISA, PCR or DIFT. They are also recommended when ASF has already been confirmed by other methods, particularly in case of a primary outbreak or case of ASF.
ASF viruses isolated in pig macrophages can be used for virus characterisation and molecular epidemiology.

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Source: https://www.legislation.gov.uk/eudn/2003/422/annex/chapter/VI/division/B/division/4 (the official version)
Page: https://openlegislation.uk/eu-decision-2003-422/annex/chapter-VI/division-B/division-4
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.

