# 2003/422/EC: Commission Decision of 26 May 2003 approving an African swine fever diagnostic manual (Text with EEA relevance) (notified under document number C(2003) 1696), Division 4

Extent: E+W+S+N.I.

The IIFT is a rapid technique with high sensitivity and specificity for the detection of ASF antibodies from either sera or tissue exudates. It is based on the detection of ASF antibodies that bind to a monolayer of MS cells infected with an adapted ASF virus. The antibody-antigen reaction is detected by a labelled fluorescein A-protein. Positive samples show specific fluorescence near the nucleus of the infected cells.
DIFT and IIFT used in combination to test organ, blood and exudates collected from animals showing clinical signs of the ASF may lead to a rapid and reliable confirmation of the disease.

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Source: https://www.legislation.gov.uk/eudn/2003/422/annex/chapter/VII/division/B/division/4 (the official version)
Page: https://openlegislation.uk/eu-decision-2003-422/annex/chapter-VII/division-B/division-4
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.