{
  "url": "https://openlegislation.uk/eu-decision-2003-422/annex/chapter-VIII",
  "json_url": "https://openlegislation.uk/eu-decision-2003-422/annex/chapter-VIII.json",
  "legislation": "eudn/2003/422",
  "legislation_title": "2003/422/EC: Commission Decision of 26 May 2003 approving an African swine fever diagnostic manual (Text with EEA relevance) (notified under document number C(2003) 1696)",
  "legislation_url": "https://openlegislation.uk/eu-decision-2003-422",
  "path": "annex/chapter/VIII",
  "kind": "chapter",
  "number": "VIII",
  "heading": "Minimum safety requirements for ASF laboratories",
  "text": "Table 1\nPrinciples of biological containment appropriate for diagnostic laboratories\nMinimum requirements\nAdditional requirements\nGeneral environment\nNormal atmospheric pressure.\nDedicated rooms limited to defined proce- dures.\nNormal atmospheric pressure. One HEPA filtration of exhaust air.\nDedicated rooms, used exclusively for clas- sical swine fever or ASF diagnostic proce- dures.\nPotentially contaminated effluents treated to inactivate ASF virus (heat or chemical).\nLaboratory clothing\nDedicated outer clothing used only in the ASF virus unit.\nDisposable gloves for all manipulations of infected material.\nOuter clothing sterilised before removal from unit, or washed at a high tempera- ture within unit.\nComplete change of clothes on entry. Laboratory clothing used only in the ASF virus unit.\nDisposable gloves for all manipulations of infected material.\nClothing sterilised before removal from unit, or washed at a high temperature within unit.\nControl of personnel\nEntry to unit limited to named, trained personnel.\nWash and disinfect hands on leaving unit.\nPersonnel not permitted to visit premises with pigs for 48 hours after leaving unit.\nEntry to unit limited to named, trained personnel.\nWash and disinfect hands on leaving unit.\nPersonnel not permitted to visit premises with pigs for 48 hours after leaving unit.\nEquipment\nBiological safety cabinet (class I or II) used for all manipulations of live virus. Cabinet should have double HEPA filtration of exhaust air.\nAll equipment needed for laboratory procedures to be available within the dedicated laboratory suite.\nTable 2\nBio-safety requirements for experimental animal rooms\nRequirements\nGeneral environment\nNegative-pressure-controlled ventilation.\nOne HEPA filtration of exhaust air.\nFacility for complete decontamination or fumigation at end of experiment.\nAll solid and liquid waste effluents treated to inactivate ASF virus (heat/incineration or chemical).\nLaboratory clothing\nComplete change of clothes on entry.\nClothing sterilised before removal from unit, or washed at a high temperature within unit.\nControl of personnel\nEntry to unit limited to named, trained personnel.\nLeave clothes inside before shower. Full shower on exit from unit.\nPersonnel not permitted to visit premises with pigs for 48 hours after leaving unit.\nEquipment\nAll equipment required for animal procedures to be available within the unit.\nAll materials to be sterilised on removal from unit or, in the case of animal samples, to be double-wrapped in leakproof container which is surface disinfected for transport to the ASF laboratory.\nAnimals\nAll animals to be slaughtered before leaving the unit, post-mortem examinations to be completed within the bio-safe area, and carcases incinerated on completion of examina- tions.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudn/2003/422/annex/chapter/VIII",
    "retrieved_at": "2026-11-01T15:18:22.461284+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}