# Commission Decision of 4 August 2006 approving a Diagnostic Manual for avian influenza as provided for in Council Directive 2005/94/EC (notified under document number C(2006) 3477) (Text with EEA relevance) (2006/437/EC), Division 7: Brain-heart-infusion medium

Extent: E+W+S+N.I.

The solution must be prepared in water and contain 15 % w/v brain-heart-infusion broth powder, prior to sterilisation (by autoclaving at 121 oC/15 minutes).
Following sterilisation antibiotics must be added as follows: 10 000 IU/ml penicillin G, 20 μg amphotericin B and 1 000 μg/ml gentamycin. Media may be stored at 4 oC for a maximum of two months.

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Source: https://www.legislation.gov.uk/eudn/2006/437/annex/chapter/IV/division/7 (the official version)
Page: https://openlegislation.uk/eu-decision-2006-437/annex/chapter-IV/division-7
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.