Division 2 Samples for virus isolation

AI virus infections in pigs are usually restricted to the respiratory tract and samples must be respiratory tract tissues and, if appropriate, oropharyngeal or nasal swabs, preferably taken from pigs showing signs of that disease. These samples and swabs may be processed for virus isolation or molecular detection of virus, using the same techniques described above for samples from birds. However, when using PCR techniques, proper controls must be used to ensure that the amplification is not inhibited by substances in the samples from pigs.