{
  "url": "https://openlegislation.uk/eu-decision-2006-668/annex-I/division-4/division-4.1",
  "json_url": "https://openlegislation.uk/eu-decision-2006-668/annex-I/division-4/division-4.1.json",
  "legislation": "eudn/2006/668",
  "legislation_title": "Commission Decision of 29 September 2006 concerning a financial contribution from the Community towards a baseline survey on the prevalence of Salmonella in slaughter pigs to be carried out in the Member States (notified under document number C(2006) 4306) (2006/668/EC)",
  "legislation_url": "https://openlegislation.uk/eu-decision-2006-668",
  "path": "annex/I/division/4/division/4.1",
  "kind": "division",
  "number": "4.1",
  "heading": "Sample preparation for bacteriology",
  "text": "Lymph nodes shall be surface de-contaminated before analysis by dipping into absolute alcohol and drying by air.\nAll lymph nodes shall be pooled and closed in a plastic bag and banged with a hammer or so on the plastic bag smashing the lymph nodes.\nThe homogenised lymph nodes shall be weighed and placed in a sterile container with pre-warmed buffered peptone water (BPW) in dilution 1:10. Containers shall be incubated for a total of (18 ± 2) hours at (37 ± 1) °C.\nRegarding swabs sample, at the laboratory, 100 ml of BPW shall be added for pre-enrichment. The sample shall be incubated at 37 °C overnight and examined for Salmonella using the modified semi-solid Rappaport-Vassiladis medium (MRSV) method (draft Annex D of the ISO 6579:2002(E).",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudn/2006/668/annex/I/division/4/division/4.1",
    "retrieved_at": "2026-11-01T15:17:28.914298+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}