# Commission Decision of 29 September 2006 concerning a financial contribution from the Community towards a baseline survey on the prevalence of Salmonella in slaughter pigs to be carried out in the Member States (notified under document number C(2006) 4306) (2006/668/EC), Division 4.1: Sample preparation for bacteriology

Extent: E+W+S+N.I.

Lymph nodes shall be surface de-contaminated before analysis by dipping into absolute alcohol and drying by air.
All lymph nodes shall be pooled and closed in a plastic bag and banged with a hammer or so on the plastic bag smashing the lymph nodes.
The homogenised lymph nodes shall be weighed and placed in a sterile container with pre-warmed buffered peptone water (BPW) in dilution 1:10. Containers shall be incubated for a total of (18 ± 2) hours at (37 ± 1) °C.
Regarding swabs sample, at the laboratory, 100 ml of BPW shall be added for pre-enrichment. The sample shall be incubated at 37 °C overnight and examined for Salmonella using the modified semi-solid Rappaport-Vassiladis medium (MRSV) method (draft Annex D of the ISO 6579:2002(E).

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Source: https://www.legislation.gov.uk/eudn/2006/668/annex/I/division/4/division/4.1 (the official version)
Page: https://openlegislation.uk/eu-decision-2006-668/annex-I/division-4/division-4.1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.