# Commission Decision of 30 March 2007 concerning a Community financial contribution towards a baseline survey on the prevalence of Salmonella in slaughter pigs to be carried out in Bulgaria and in Romania (notified under document number C(2007) 1394) (Only the Bulgarian and Romanian texts are authentic) (2007/219/EC), Division 4.1: Sample preparation

Extent: E+W+S+N.I.

Lymph nodes shall be surface de-contaminated before analysis by dipping into absolute alcohol and drying by air.
All lymph nodes shall be pooled and closed in a plastic bag and banged with a hammer or by similar means on the plastic bag smashing the lymph nodes.
The homogenized lymph nodes shall be weighed and placed in a sterile container with pre-warmed buffered peptone water (BPW) in dilution 1:10. Containers shall be incubated for a total of (18 ± 2) hours at (37 ± 1) °C.

---
Source: https://www.legislation.gov.uk/eudn/2007/219/annex/I/division/4/division/4.1 (the official version)
Page: https://openlegislation.uk/eu-decision-2007-219/annex-I/division-4/division-4.1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.