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Division 2.3 Initial suspension

United Kingdom

Original text. Later amendments are not shown.

The 27 g test portion shall be transferred to NINE volumes (243 ml) buffered peptone water (BPW), brought to room temperature before adding. The mixture shall be treated in a stomacher or pulsifier for approximately ONE minute (27 g are needed to perform analyses for Salmonella spp. and Campylobacter spp. from one sample in parallel). Foaming shall be avoided by removing the air from the stomacher bag as much as possible.

This initial suspension shall be used as follows:

  1. (a)

    10 ml (~1g) shall be transferred to 90 ml enrichment medium for Campylobacter spp. detection;

  2. (b)

    10 ml (~1g) shall be transferred to an empty sterile tube; 1 ml is used for the enumeration of Campylobacter spp. on selective plates.

The rest of the initial suspension (250 ml ~ 25g) shall be used for the detection of Salmonella spp.