Division III.2 Official investigation and diagnostic methods to rule out and to confirm the presence of infection with KHV

When a suspicion of KHVD is required to be confirmed or ruled out in accordance with F1Part 4 of the Aquatic Animal Health Regulations, the following inspection, sampling and testing procedure shall be complied with:

1. (a)

   the official investigation shall include at least one health inspection and one sampling of 10 fish, when clinical signs or post-mortem signs consistent with infection with KHV are observed or 30 fish when clinical or post-mortem signs are not observed. Samples shall be tested using the diagnostic method set out in point (b) in accordance with the detailed diagnostic methods and procedures set out in point II of Part 2 of Annex II;

2. (b)

   the presence of the infection with KHV shall be considered as confirmed if KHV is detected by PCR;

   suspicion of KHVD may be ruled out, if this test reveals no further evidence of the presence of KHV.