{
  "url": "https://openlegislation.uk/eu-decision-2015-1554/annex-I/part-3/division-II/division-II.2",
  "json_url": "https://openlegislation.uk/eu-decision-2015-1554/annex-I/part-3/division-II/division-II.2.json",
  "legislation": "eudn/2015/1554",
  "legislation_title": "Commission Implementing Decision (EU) 2015/1554 of 11 September 2015 laying down rules for the application of Directive 2006/88/EC as regards requirements for surveillance and diagnostic methods (notified under document C(2015) 6188) (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-decision-2015-1554",
  "path": "annex/I/part/3/division/II/division/II.2",
  "kind": "division",
  "number": "II.2",
  "heading": "Diagnostic methods to obtain or maintain disease-free status with regard to ISA",
  "text": "The diagnostic method to be used to obtain or to maintain disease-free status with regard to ISA in accordance with points I.2 and I.3 shall be RT-qPCR, followed by sequencing of positive samples in accordance with the detailed methods and procedures set out in Part 3 of Annex II.\nIn the case of a positive result to RT-qPCR, further samples shall be tested before the implementation of the initial control measures provided for in Part 4 of the Aquatic Animal Health Regulations.\nThose samples shall be tested as follows in accordance with the detailed methods and procedures set out in Part 3 of Annex II:\n(a)screening of the samples by RT-qPCR, including sequencing of the HE-gene to verify HPR-deletion;\nand\n(b)examination in tissue preparations by means of specific antibodies against ISAV (namely IHC on fixed sections or IFAT on tissue imprints); or\n(c)isolation and identification of ISAV in cell culture from at least one sample from any fish sampled from the farm.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Words in Annex 1 Pt. 3 para. II.2 substituted (E.W.S.) (31.12.2020) by The Animals, Aquatic Animal Health, Invasive Alien Species, Plant Propagating Material and Seeds (Amendment) (EU Exit) Regulations 2020 (S.I. 2020/1388), regs. 1(2)(c), 11(14)(h)"
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudn/2015/1554/annex/I/part/3/division/II/division/II.2",
    "retrieved_at": "2026-11-01T15:14:14.330569+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}