# Commission Implementing Decision (EU) 2015/1554 of 11 September 2015 laying down rules for the application of Directive 2006/88/EC as regards requirements for surveillance and diagnostic methods (notified under document C(2015) 6188) (Text with EEA relevance), Division II.2: Diagnostic methods to obtain or maintain disease-free status with regard to ISA

Extent: E+W+S+N.I.

The diagnostic method to be used to obtain or to maintain disease-free status with regard to ISA in accordance with points I.2 and I.3 shall be RT-qPCR, followed by sequencing of positive samples in accordance with the detailed methods and procedures set out in Part 3 of Annex II.
In the case of a positive result to RT-qPCR, further samples shall be tested before the implementation of the initial control measures provided for in Part 4 of the Aquatic Animal Health Regulations.
Those samples shall be tested as follows in accordance with the detailed methods and procedures set out in Part 3 of Annex II:
(a)screening of the samples by RT-qPCR, including sequencing of the HE-gene to verify HPR-deletion;
and
(b)examination in tissue preparations by means of specific antibodies against ISAV (namely IHC on fixed sections or IFAT on tissue imprints); or
(c)isolation and identification of ISAV in cell culture from at least one sample from any fish sampled from the farm.

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Source: https://www.legislation.gov.uk/eudn/2015/1554/annex/I/part/3/division/II/division/II.2 (the official version)
Page: https://openlegislation.uk/eu-decision-2015-1554/annex-I/part-3/division-II/division-II.2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.