# Commission Implementing Decision (EU) 2015/1554 of 11 September 2015 laying down rules for the application of Directive 2006/88/EC as regards requirements for surveillance and diagnostic methods (notified under document C(2015) 6188) (Text with EEA relevance), Division I.4.4: Sample preparation for RT-PCR and RT-qPCR based surveillance programmes

Extent: E+W+S+N.I.

If samples were collected in transport medium, the procedure set out in points I.4.2 and I.4.3 shall be carried out. After centrifugation, supernatant shall be collected and RNA extracted. If further examination is not to be undertaken directly after centrifugation, the samples shall be immediately frozen at – 20 °C or below.
For the analysis of fish tissues preserved in RNA stabilization reagent, subsequent work shall be carried out within the following time scales for samples stored at different temperatures:
samples stored at 37 °C: 1 day;
samples stored at 25 °C: 1 week;
samples stored at 4 °C: 1 month;
samples stored at – 20 °C: indefinitely
Pooled samples in RNA stabilization reagent shall be treated like single samples in RNA stabilization reagent. For samples pooled in RNA stabilization reagent, the sample amount shall not exceed that recommended by the manufacturer for extraction with RNA kits, such as RNeasy Mini kits (Qiagen) or similar. If larger samples are pooled, the extraction kits or methods must reflect this pooling.
Samples collected in RNA stabilization reagents shall not be used for cell cultivation.

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Source: https://www.legislation.gov.uk/eudn/2015/1554/annex/II/part/1/division/I/division/I.4/division/I.4.4 (the official version)
Page: https://openlegislation.uk/eu-decision-2015-1554/annex-II/part-1/division-I/division-I.4/division-I.4.4
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.