Division II.2 Surveillance of KHVD by PCR based methods

For the surveillance of KHV, a qPCR shall be used. If positive samples appear in an area not previously confirmed positive, the test results shall be confirmed either:

1. (a)

   by sequencing of a PCR or nested PCR product from the samples.

   The obtained clean consensus sequence shall match (by at least 98 %) with these reference sequences.

2. (b)

   or alternatively, samples may be sent to a national reference laboratory for confirmation.