OpenLegislation

Division II.1 Examination of samples by RT-PCR

United Kingdom

Original text. Later amendments are not shown.

The diagnostic method to be used for the screening for ISAV shall be RT-qPCR. As the results of the RT-qPCR can vary depending on the conditions under which it is performed, adequate positive and negative controls and amplicons shall be included to avoid any doubts.

  1. II.1.1Total RNA extraction
  2. II.1.2RT-PCR for ISAV detection
  3. II.1.3RT-qPCR for ISAV detection
  4. II.1.4Sequencing of amplified PCR products