{
  "url": "https://openlegislation.uk/eu-decision-2015-1554/annex-II/part-3/division-II/division-II.4",
  "json_url": "https://openlegislation.uk/eu-decision-2015-1554/annex-II/part-3/division-II/division-II.4.json",
  "legislation": "eudn/2015/1554",
  "legislation_title": "Commission Implementing Decision (EU) 2015/1554 of 11 September 2015 laying down rules for the application of Directive 2006/88/EC as regards requirements for surveillance and diagnostic methods (notified under document C(2015) 6188) (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-decision-2015-1554",
  "path": "annex/II/part/3/division/II/division/II.4",
  "kind": "division",
  "number": "II.4",
  "heading": "Histology",
  "text": "Paraffin-embedded sections shall be cut at 5 μm and stained using haematoxylin and eosin.\nHistological changes in clinically diseased Atlantic salmon are variable, but may include the following:\n(a)numerous erythrocytes in the central venous sinus and lamellar capillaries of the gills, where erythrocyte thrombi also may be formed;\n(b)multifocal to confluent petechiae or hepatocyte necrosis or both at some distance from larger vessels in the liver; multifocal accumulation of erythrocytes in dilated hepatic sinusoids;\n(c)accumulation of erythrocytes in blood vessels of the intestinal lamina propria and eventually haemorrhage into the lamina propria;\n(d)spleen stroma distended by erythrocyte accumulation;\n(e)slight multifocal to extensive diffuse interstitial haemorrhage with tubular necrosis in the haemorrhagic areas, erythrocyte accumulation in the glomeruli in the kidney;\n(f)erythrophagocytosis in the spleen and secondary haemorrhages in liver and kidney.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudn/2015/1554/annex/II/part/3/division/II/division/II.4",
    "retrieved_at": "2026-11-01T15:14:14.330569+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}