{
  "url": "https://openlegislation.uk/eu-decision-2015-1554/annex-II/part-6/division-4",
  "json_url": "https://openlegislation.uk/eu-decision-2015-1554/annex-II/part-6/division-4.json",
  "legislation": "eudn/2015/1554",
  "legislation_title": "Commission Implementing Decision (EU) 2015/1554 of 11 September 2015 laying down rules for the application of Directive 2006/88/EC as regards requirements for surveillance and diagnostic methods (notified under document C(2015) 6188) (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-decision-2015-1554",
  "path": "annex/II/part/6/division/4",
  "kind": "division",
  "number": "4",
  "heading": "DNA extraction",
  "text": "Total DNA shall be extracted using a DNA tissue extraction kit and the EZ1 Advanced XL Biorobot (Qiagen) following the manufacturers' instructions. An extraction control (Calf Thymus DNA) and a negative control (G2 buffer) shall run with each batch of samples. DNA shall be eluted into 50 μl volume. To ensure that the extraction has completed successfully, the DNA concentration for all samples and controls shall be determined using a Nano Drop machine. Extracted DNA shall be frozen at – 20 °C if not required immediately.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudn/2015/1554/annex/II/part/6/division/4",
    "retrieved_at": "2026-11-01T15:14:14.330569+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}