Division Laboratory Criteria

At least one of the following:

• Demonstration of Treponema pallidum in lesion exudates or tissues by dark-field microscopic examination

• Demonstration of Treponema pallidum in lesion exudates or tissues by DFA test

• Demonstration of Treponema in lesion exudates or tissues by nuclear acid amplification techniques (NAAT)

• Detection of Treponema pallidum antibodies by screening test (TPHA, TPPA or EIA) AND additionally detection of either TP-IgM antibodies (for example, IgM-ELISA or immunoblot or 19S-IgM-FTA-abs) OR non-TP antibodies (for example, RPR, VDRL).