{
  "url": "https://openlegislation.uk/eu-decision-2020-1729/annex/part-A/division-4/division-4.2",
  "json_url": "https://openlegislation.uk/eu-decision-2020-1729/annex/part-A/division-4/division-4.2.json",
  "legislation": "eudn/2020/1729",
  "legislation_title": "Commission Implementing Decision (EU) 2020/1729 of 17 November 2020 on the monitoring and reporting of antimicrobial resistance in zoonotic and commensal bacteria and repealing Implementing Decision 2013/652/EU (notified under document C(2020) 7894) (Only the English version is authentic) (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-decision-2020-1729",
  "path": "annex/part/A/division/4/division/4.2",
  "kind": "division",
  "number": "4.2",
  "heading": "Analytical methods for detection and antimicrobial susceptibility testing",
  "text": "Member States shall use the epidemiological cut-off values and the concentration ranges set out in Tables 2, 3 and 4 below to determine the antimicrobial susceptibility of Salmonella spp., C. coli, C. jejuni, indicator commensal E. coli, E. faecalis and E. faecium.\nAny E. coli and Salmonella isolate tested in accordance with Table 2 showing resistance to cefotaxime or ceftazidime or meropenem shall be further tested with a second panel of antimicrobial substances in accordance with Table 5.\nFor the specific monitoring of ESBL-, AmpC- and/or CP-producing E. coli, Member States shall use the methods referred to in point 5.\nThe antimicrobial susceptibility testing shall be performed by the laboratories referred to in Article 3(2). The testing shall be performed by using the broth micro dilution method according to the reference method ISO 20776-1:2019.\nTable 2\nPanel of antimicrobial substances to be included in AMR monitoring, EUCAST thresholds for resistance and concentration ranges to be tested in Salmonella spp. and indicator commensal E. coli (First panel)\ntentative EUCAST threshold\nNA: not available.\nAntimicrobial\nClass of antimicrobial\nSpecies\nInterpretative thresholds of AMR (mg/L)\nRange of concentrations (mg/L)(No of wells in brackets)\nECOFF\nClinical breakpoint\nAmikacin\nAminoglycoside\nSalmonella\n> 4\n> 16\n4-128 (6)\nE. coli\n> 8\n> 16\nAmpicillin\nPenicillin\nSalmonella\n> 8\n> 8\n1-32 (6)\nE. coli\n> 8\n> 8\nAzithromycin\nMacrolide\nSalmonella\nNA\nNA\n2-64 (6)\nE. coli\nNA\nNA\nCefotaxime\nCephalosporin\nSalmonella\n> 0,5\n> 2\n0,25-4 (5)\nE, coli\n> 0,25\n> 2\nCeftazidime\nCephalosporin\nSalmonella\n> 2\n> 4\n0,25-8 (6)\nE, coli\n> 0,5\n> 4\nChloramphenicol\nPhenicol\nSalmonella\n> 16\n> 8\n8-64 (4)\nE, coli\n> 16\n> 8\nCiprofloxacin\nFluoroquinolone\nSalmonella\n> 0,06\n> 0,06\n0,015-8 (10)\nE, coli\n> 0,06\n> 0,5\nColistin\nPolymyxin\nSalmonella\nNA\n> 2\n1-16 (5)\nE, coli\n> 2\n> 2\nGentamicin\nAminoglycoside\nSalmonella\n> 2\n> 4\n0,5-16 (6)\nE, coli\n> 2\n> 4\nMeropenem\nCarbapenem\nSalmonella\n> 0,125\n> 8\n0,03-16 (10)\nE, coli\n> 0,125\n> 8\nNalidixic acid\nQuinolone\nSalmonella\n> 8\nNA\n4-64 (5)\nE, coli\n> 8\nNA\nSulfamethoxazole\nFolate pathway antagonist\nSalmonella\nNA\nNA\n8-512 (7)\nE, coli\n> 64\nNA\nTetracycline\nTetracycline\nSalmonella\n> 8\nNA\n2-32 (5)\nE, coli\n> 8\nNA\nTigecycline\nGlycylcycline\nSalmonella\nNA\nNA\n0,25-8 (6)\nE, coli\n> 0,5\n> 0,5\nTrimethoprim\nFolate pathway antagonist\nSalmonella\n> 2\n> 4\n0,25-16 (7)\nE, coli\n> 2\n> 4\nTable 3\nPanel of antimicrobial substances to be included in AMR monitoring, EUCAST interpretative thresholds for resistance and concentration ranges to be tested in C. jejuni and C. coli\nNA: not available\nAntimicrobial\nClass of antimicrobial\nSpecies\nInterpretative thresholds of AMR (mg/L)\nRange of concentrations (mg/L)(No of wells in brackets)\nECOFF\nClinical breakpoint\nChloramphenicol\nPhenicol\nC. jejuni\n> 16\nNA\n2-64 (6)\nC. coli\n> 16\nNA\nCiprofloxacin\nFluoroquinolone\nC. jejuni\n> 0,5\n> 0,5\n0,12-32 (9)\nC. coli\n> 0,5\n> 0,5\nErtapenem\nCarbapenem\nC. jejuni\nNA\nNA\n0,125-4 (6)\nC. coli\nNA\nNA\nErythromycin\nMacrolide\nC. jejuni\n> 4\n> 4\n1-512 (10)\nC. coli\n> 8\n> 8\nGentamicin\nAminoglycoside\nC. jejuni\n> 2\nNA\n0,25-16 (7)\nC. coli\n> 2\nNA\nTetracycline\nTetracycline\nC. jejuni\n> 1\n> 2\n0,5-64 (8)\nC. coli\n> 2\n> 2\nTable 4\nPanel of antimicrobial substances to be included in AMR monitoring, EUCAST thresholds for resistance and concentration ranges to be tested in E. faecalis and E. faecium\nNA: not available\nAntimicrobial\nClass of antimicrobial\nSpecies\nInterpretative thresholds of AMR (mg/L)\nRange of concentrations (mg/L)(No of wells in brackets)\nECOFF\nClinical breakpoint\nAmpicillin\nPenicillin\nE. faecalis\n> 4\n> 8\n0,5-64 (8)\nE. faecium\n> 4\n> 8\nChloramphenicol\nPhenicol\nE. faecalis\n> 32\nNA\n4-128 (6)\nE. faecium\n> 32\nNA\nCiprofloxacin\nFluoroquinolone\nE. faecalis\n> 4\n> 4\n0,12-16 (8)\nE. faecium\n> 4\n> 4\nDaptomycin\nLipopeptide\nE. faecalis\n> 4\nNA\n0,25-32 (8)\nE. faecium\n> 8\nNA\nErythromycin\nMacrolide\nE. faecalis\n> 4\nNA\n1-128 (8)\nE. faecium\n> 4\nNA\nGentamicin\nAminoglycoside\nE. faecalis\n> 64\nNA\n8-1 024 (8)\nE. faecium\n> 32\nNA\nLinezolid\nOxazolidinone\nE. faecalis\n> 4\n> 4\n0,5-64 (8)\nE. faecium\n> 4\n> 4\nQuinupristin/Dalfopristin\nStreptogramin\nE. faecalis\nNA\nNA\n0,5-64 (8)\nE. faecium\nNA\n> 4\nTeicoplanin\nGlycopeptide\nE. faecalis\n> 2\n> 2\n0,5-64 (8)\nE. faecium\n> 2\n> 2\nTetracycline\nTetracycline\nE. faecalis\n> 4\nNA\n1-128 (8)\nE. faecium\n> 4\nNA\nTigecycline\nGlycylcycline\nE. faecalis\n> 0,25\n> 0,25\n0,03-4 (8)\nE. faecium\n> 0,25\n> 0,25\nVancomycin\nGlycopeptide\nE. faecalis\n> 4\n> 4\n1-128 (8)\nE. faecium\n> 4\n> 4",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "footnote",
      "label": "f1",
      "text": "tentative EUCAST threshold"
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudn/2020/1729/annex/part/A/division/4/division/4.2",
    "retrieved_at": "2026-11-01T15:12:05.159169+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}