# Commission Implementing Decision (EU) 2020/1729 of 17 November 2020 on the monitoring and reporting of antimicrobial resistance in zoonotic and commensal bacteria and repealing Implementing Decision 2013/652/EU (notified under document C(2020) 7894) (Only the English version is authentic) (Text with EEA relevance), Division 5.1: Methods for detection of presumptive ESBL- or AmpC- or CP-producing E. coli

Extent: E+W+S+N.I.

For the purpose of estimating the proportion of samples containing presumptive ESBL- or AmpC- or CP-producing E. coli among the caecal and fresh meat samples collected in accordance with point 1(d), the laboratories referred to in Article 3(2) shall use detection methods detailed in the protocols of the EURL for AMR.
All presumptive ESBL- or AmpC- or CP-producing E. coli isolates identified through the methods referred to in above shall be tested with the first panel and the second panel of antimicrobial substances in accordance with Table 2 and Table 5 respectively.
Table 5
Panel of antimicrobial substances, EUCAST epidemiological cut-off values (ECOFFs) and clinical resistance breakpoints and concentrations ranges to be used for testing only Salmonella spp. and E. coli isolates resistant to cefotaxime or ceftazidime or meropenem – (Second panel)
NA: not available
Antimicrobial
Class of antimicrobial
Species
Interpretative thresholds of AMR (mg/L)
Range of concentrations (mg/L)(No of wells in brackets)
ECOFF
Clinical breakpoint
Cefepime
Cephalosporin
Salmonella
NA
> 4
0,06-32 (10)
E. coli
> 0,125
> 4
Cefotaxime
Cephalosporin
Salmonella
> 0,5
> 2
0,25-64 (9)
E. coli
> 0,25
> 2
Cefotaxime + clavulanic acid
Cephalosporin/beta-lactamase inhibitor combination
Salmonella
NA
NA
0,06-64 (11)
E. coli
> 0,25
NA
Cefoxitin
Cephamycin
Salmonella
> 8
NA
0,5-64 (8)
E. coli
> 8
NA
Ceftazidime
Cephalosporin
Salmonella
> 2
> 4
0,25-128 (10)
E. coli
> 0,5
> 4
Ceftazidime + clavulanic acid
Cephalosporin//beta-lactamase inhibitor combination
Salmonella
NA
NA
0,125-128 (11)
E. coli
> 0,5
NA
Ertapenem
Carbapenem
Salmonella
NA
> 0,5
0,015-2 (8)
E. coli
NA
> 0,5
Imipenem
Carbapenem
Salmonella
> 1
> 4
0,12-16 (8)
E. coli
> 0,5
> 4
Meropenem
Carbapenem
Salmonella
> 0,125
> 8
0,03-16 (10)
E. coli
> 0,125
> 8
Temocillin
Penicillin
Salmonella
> NA
NA
0,5-128 (9)
E. coli
> 16
NA

---
Source: https://www.legislation.gov.uk/eudn/2020/1729/annex/part/A/division/5/division/5.1 (the official version)
Page: https://openlegislation.uk/eu-decision-2020-1729/annex/part-A/division-5/division-5.1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.