# Commission Implementing Decision (EU) 2020/569 of 16 April 2020 establishing a common format and information content for the submission of the information to be reported by Member States pursuant to Directive 2010/63/EU of the European Parliament and of the Council on the protection of animals used for scientific purposes and repealing Commission Implementing Decision 2012/707/EU (notified under document C(2020) 2179) (Text with EEA relevance), Division 2.2: Reuse versus continued use

Extent: E+W+S+N.I.

For the purposes of determining whether there is a ‘reuse’, the following shall apply:
2.2.1. A single use is the use of one animal for a single scientific/experimental/educational/training purpose. A single use extends from the time when the first technique is applied to the animal until the completion of data collection, observations or achievement of educational objective. This is usually a single experiment, test or training of a technique.
2.2.2. A single use may contain a number of steps (techniques) all necessarily related to achieve a single outcome and which require the use of the same animal.
2.2.3. Examples of preparation for the purposes of continued use include:
(a) surgical techniques (such as cannulation, implantation of telemetry, ovariectomy, castration, hypophysectomy);
(b) non-surgical techniques (such as feeding modified diets, induction of diabetes, induction of transgene expression);
(c) breeding of genetically altered animals of harmful phenotype;
(d) genetic characterisation using an invasive method (which was not carried out for the purposes of identification/marking of the animal) and where an animal of that genotype is required for the next step.
2.2.4. When the prepared animal is used in the procedure intended for it, the entire procedure, including any preparation (regardless of the location this has taken place) is reported at the end taking into account the severity associated with the preparation. For example, for the breeding of a genetically altered animal and its end use, the reporting shall take into account the severity associated with all the steps (for example, the effect of the phenotype, if expressed; genetic characterisation, if performed; and end use).
2.2.5. The use of an animal is only reported once at the end of the complete procedure including where the preparatory steps described in point 2.2.3 and the end use have been carried out under separate projects.
2.2.6. Where a prepared animal is not subsequently used for a scientific purpose, the establishment in which the animal is killed shall report the preparation as an independent use in the statistics as per the intended purpose, provided that the preparation of the animal has been above the threshold of minimum pain, suffering, distress and lasting harm. However, if this preparation concerns maintenance of a genetically altered animal line, the criteria by which animals are reported are provided for in section B.10.7.
2.2.7. If the animal has been genotyped (genetic characterisation/tissue sampling) as part of a routine verification in a genetically altered breeding colony of an established line to confirm that the genotype has not varied from the intended genetic background and that animal is later used in another procedure, not requiring that particular genotype, that use is considered reuse and all such uses shall be reported separately in the statistics, that is to say:
(a) first use under ‘maintenance of the established genetically altered line’ with the severity related to the actual severity experienced by the animal as the result of the invasive genotyping, and
(b) as reuse under the specific purpose the animal is used for.

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Source: https://www.legislation.gov.uk/eudn/2020/569/annex/III/part/B/division/B/division/2/division/2.2 (the official version)
Page: https://openlegislation.uk/eu-decision-2020-569/annex-III/part-B/division-B/division-2/division-2.2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.