Division 1 A bovine herd is officially brucellosis-free if:F1F2

it contains no bovine animals which have been vaccinated against brucellosis, except females which have been vaccinated at least three years previously;

all the bovine animals have been free from clinical signs of brucellosis for at least six months;

all the bovine animals over 12 months old have been subjected to one of the following test regimes with negative results in accordance with Annex C:

1. (i)

   two serological tests specified in paragraph 10 at an interval of more than three months and less than 12 months;

2. (ii)

   three tests on milk samples at three-monthly intervals followed at least six weeks later by a serological test specified in paragraph 10;

any bovine animal entering the herd comes from a herd of officially brucellosis-free status and, in the case of bovine animals over 12 months old, has shown a brucella titre of less than 30 IU of agglutination per ml when given a serum agglutination test in accordance with Annex C or has reacted negatively to any other test approved in accordance with the procedure at Article 17 during the 30 days prior to or the 30 days after the date of its introduction into the herd: in the latter case, the animal(s) must be isolated physically from the other animals of the herd in such a way as to avoid direct or indirect contact with the other animals until proven negative.