{
  "url": "https://openlegislation.uk/eu-directive-1964-432/annex-C/division-2/division-2.2/division-2.2.2/division-2.2.2.1",
  "json_url": "https://openlegislation.uk/eu-directive-1964-432/annex-C/division-2/division-2.2/division-2.2.2/division-2.2.2.1.json",
  "legislation": "eudr/1964/432",
  "legislation_title": "Council Directive of 26 June 1964 on animal health problems affecting intra-Community trade in bovine animals and swine (64/432/EEC)",
  "legislation_url": "https://openlegislation.uk/eu-directive-1964-432",
  "path": "annex/C/division/2/division/2.2/division/2.2.2/division/2.2.2.1",
  "kind": "division",
  "number": "2.2.2.1",
  "heading": null,
  "text": "Standardisation of the test procedure for individual serum samples:\n(a) a 1/150 pre-dilution of the OIEISS or a 1/2 pre-dilution of the OIEELISA WP SS or a 1/16 pre-dilution of the OIEELISA SP SS made up in a negative serum (or in a negative pool of sera) must give a positive reaction;\n(b) a 1/600 pre-dilution of the OIEISS or a 1/8 pre-dilution of the OIEELISA WP SS or a 1/64 pre-dilution of the OIEELISA SP SS made up in a negative serum (or in a negative pool of sera) must give a negative reaction;\n(c) the OIEELISA N SS must always give a negative reaction.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Substituted by Council Directive 97/12/EC of 17 March 1997 amending and updating Directive 64/432/EEC on health problems affecting intra-Community trade in bovine animals and swine."
    },
    {
      "type": "F",
      "label": "F2",
      "text": "Substituted by Commission Regulation (EC) No 535/2002 of 21 March 2002 amending Annex C to Council Directive 64/432/EEC and amending Decision 2000/330/EC."
    },
    {
      "type": "F",
      "label": "F3",
      "text": "Substituted by Commission Decision of 10 December 2008 amending Annex C to Council Directive 64/432/EEC and Decision 2004/226/EC as regards diagnostic tests for bovine brucellosis (notified under document number C(2008) 7642) (Text with EEA relevance) (2008/984/EC)."
    },
    {
      "type": "footnote",
      "label": "f1",
      "text": "For the purpose of this Annex, dilutions given for making up liquid reagents are expressed as, for example, 1/150 shall mean a 1 in 150 dilution."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudr/1964/432/annex/C/division/2/division/2.2/division/2.2.2/division/2.2.2.1",
    "retrieved_at": "2026-11-01T15:23:56.007863+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}