# Council Directive of 26 June 1964 on animal health problems affecting intra-Community trade in bovine animals and swine (64/432/EEC), Division 2.2.2.3

Extent: E+W+S+N.I.

Standardisation of the test procedure for pooled milk or whey samples:
(a) a 1/ 1 000 pre-dilution of the OIEISS or a 1/16 pre-dilution of the OIEELISA WP SS or a 1/125 pre-dilution of the OIEELISA SP SS made up in a negative serum (or in a negative pool of sera) and again diluted 1/10 in negative milk must give a positive reaction;
(b) the OIEELISA N SS diluted 1/10 in negative milk must always give a negative reaction;
(c) the test must be adequate to detect evidence of infection in a single animal of the group of animals, of which samples of milk or whey have been pooled.

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Source: https://www.legislation.gov.uk/eudr/1964/432/annex/C/division/2/division/2.2/division/2.2.2/division/2.2.2.3 (the official version)
Page: https://openlegislation.uk/eu-directive-1964-432/annex-C/division-2/division-2.2/division-2.2.2/division-2.2.2.3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.