# Council Directive of 26 June 1964 on animal health problems affecting intra-Community trade in bovine animals and swine (64/432/EEC), Division 2.3.4

Extent: E+W+S+N.I.

In carrying out the complement fixation test, the following controls must be made each time:
(a) control of the anti-complementary effect of the serum;
(b) control of the antigen;
(c) control of sensitised red blood cells;
(d) control of the complement;
(e) control using a positive serum of sensitivity at the start of the reaction;
(f) control of the specificity of the reaction using a negative serum.

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Source: https://www.legislation.gov.uk/eudr/1964/432/annex/C/division/2/division/2.3/division/2.3.4 (the official version)
Page: https://openlegislation.uk/eu-directive-1964-432/annex-C/division-2/division-2.3/division-2.3.4
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.