# Council Directive of 26 June 1964 on animal health problems affecting intra-Community trade in bovine animals and swine (64/432/EEC), Division 2.5.3

Extent: E+W+S+N.I.

The RBT shall be carried out in the following manner:
(a) serum (20-30 μl) is mixed with an equal volume of antigen on a white tile or enamel plate to produce a zone approximately 2 cm in diameter. The mixture is rocked gently for four minutes at ambient temperature, and then observed in a good light for agglutination;
(b) an automated method may be used but must be at least as sensitive and accurate as the manual method.

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Source: https://www.legislation.gov.uk/eudr/1964/432/annex/C/division/2/division/2.5/division/2.5.3 (the official version)
Page: https://openlegislation.uk/eu-directive-1964-432/annex-C/division-2/division-2.5/division-2.5.3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.