# Council Directive of 26 June 1964 on animal health problems affecting intra-Community trade in bovine animals and swine (64/432/EEC), Division 2.6.3

Extent: E+W+S+N.I.

The antigen shall be prepared without reference to the cell concentration, but its sensitivity must be standardised in relation to the OIEISS in such a way that the antigen produces either a 50 % agglutination with a final serum dilution of 1/600 to 1/ 1 000 or 75 % agglutination with a final serum dilution of 1/500 to 1/750.
It may also be advisable to compare the reactivity of new and previously standardised batches of antigen using a panel of defined sera.

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Source: https://www.legislation.gov.uk/eudr/1964/432/annex/C/division/2/division/2.6/division/2.6.3 (the official version)
Page: https://openlegislation.uk/eu-directive-1964-432/annex-C/division-2/division-2.6/division-2.6.3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.