# Council Directive of 26 June 1964 on animal health problems affecting intra-Community trade in bovine animals and swine (64/432/EEC), Division 2.6.4

Extent: E+W+S+N.I.

The test shall be performed either in tubes or in microplates. The mixture of antigen and serum dilutions shall be incubated for 16- to 24-hours at 37 °C.
At least three dilutions must be prepared for each serum. Dilutions of suspect serum must be made in such a way that reading of the reaction at the positivity limit is made in the median tube (or well for the microplate method).

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Source: https://www.legislation.gov.uk/eudr/1964/432/annex/C/division/2/division/2.6/division/2.6.4 (the official version)
Page: https://openlegislation.uk/eu-directive-1964-432/annex-C/division-2/division-2.6/division-2.6.4
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.