# Council Directive of 26 June 1964 on animal health problems affecting intra-Community trade in bovine animals and swine (64/432/EEC), Division 4: The reagents of the tests shall consist of:

Extent: E+W+S+N.I.

(a) antigen: the antigen shall contain specific glycoprotein of enzootic bovine leukosis virus which has been standardised against the E05 serum;
(b) the test serum;
(c) known positive control serum;
(d) agar gel:
0,8 % agar,
8,5 % NaCl,
0,05 M Tris-buffer pH 7,2,
15 ml of this agar shall be introduced into a petri dish of 85 mm diameter, resulting in a depth of 2,6 mm of agar.

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Source: https://www.legislation.gov.uk/eudr/1964/432/annex/D/chapter/II/division/A/division/4 (the official version)
Page: https://openlegislation.uk/eu-directive-1964-432/annex-D/chapter-II/division-A/division-4
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.