# Council Directive of 26 June 1964 on animal health problems affecting intra-Community trade in bovine animals and swine (64/432/EEC), Division 1: Solutions and materials required:

Extent: E+W+S+N.I.

(a) 40 ml of 1,6 % agarose in 0,05 M Tris/HCl buffer, pH 7,2 with 8,5 % NaCl;
(b) 15 ml of a bovine leukosis serum, having antibody only to bovine leukosis virus glycoproteins, diluted 1:10 in 0,05 M Tris/HCl buffer, pH 7,2 with 8,5 % NaCl;
(c) 15 ml of a bovine leukosis serum, having antibody only to bovine leukosis virus glycoproteins, diluted 1:5 in 0,05 M Tris/HCl buffer, pH 7,2 with 8,5 % NaCl;
(d) four plastic petri dishes with a diameter of 85 mm;
(e) a punch with a diameter of 4 to 6 mm;
(f) a reference antigen;
(g) the antigen which is to be standardised;
(h) a water bath (56 °C).

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Source: https://www.legislation.gov.uk/eudr/1964/432/annex/D/chapter/II/division/B/division/1 (the official version)
Page: https://openlegislation.uk/eu-directive-1964-432/annex-D/chapter-II/division-B/division-1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.