{
  "url": "https://openlegislation.uk/eu-directive-1964-432/annex-D/chapter-II/division-B/division-4",
  "json_url": "https://openlegislation.uk/eu-directive-1964-432/annex-D/chapter-II/division-B/division-4.json",
  "legislation": "eudr/1964/432",
  "legislation_title": "Council Directive of 26 June 1964 on animal health problems affecting intra-Community trade in bovine animals and swine (64/432/EEC)",
  "legislation_url": "https://openlegislation.uk/eu-directive-1964-432",
  "path": "annex/D/chapter/II/division/B/division/4",
  "kind": "division",
  "number": "4",
  "heading": "Additional instructions:",
  "text": "(a) the experiment shall be carried out with two serum dilutions (1:5 and 1:10) in order to achieve optimal precipitation;\n(b) if the precipitation diameter is too small with both dilutions, then the serum shall be further diluted;\n(c) if the precipitation diameter in both dilutions is too large and faint, then a lower serum shall be chosen;\n(d) the final concentration of the agarose shall be 0,8 %; that of the sera 5 and 10 % respectively;\n(e) plot the measured diameters in the following coordinate system. The dilution of the antigen to be tested with the same diameter as the reference antigen is the working dilution.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Substituted by Council Directive 97/12/EC of 17 March 1997 amending and updating Directive 64/432/EEC on health problems affecting intra-Community trade in bovine animals and swine."
    },
    {
      "type": "F",
      "label": "F2",
      "text": "Substituted by Commission Decision of 15 December 2009 amending Annex D to Council Directive 64/432/EEC as regards diagnostic tests for enzootic bovine leukosis (notified under document C(2009) 9951) (Text with EEA relevance) (2009/976/EU)."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eudr/1964/432/annex/D/chapter/II/division/B/division/4",
    "retrieved_at": "2026-11-01T15:23:56.007863+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}