# Council Directive of 26 June 1964 on animal health problems affecting intra-Community trade in bovine animals and swine (64/432/EEC), Division 4: Additional instructions:

Extent: E+W+S+N.I.

(a) the experiment shall be carried out with two serum dilutions (1:5 and 1:10) in order to achieve optimal precipitation;
(b) if the precipitation diameter is too small with both dilutions, then the serum shall be further diluted;
(c) if the precipitation diameter in both dilutions is too large and faint, then a lower serum shall be chosen;
(d) the final concentration of the agarose shall be 0,8 %; that of the sera 5 and 10 % respectively;
(e) plot the measured diameters in the following coordinate system. The dilution of the antigen to be tested with the same diameter as the reference antigen is the working dilution.

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Source: https://www.legislation.gov.uk/eudr/1964/432/annex/D/chapter/II/division/B/division/4 (the official version)
Page: https://openlegislation.uk/eu-directive-1964-432/annex-D/chapter-II/division-B/division-4
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.